Digital LSR 2000 manuals

Digital LSR 2000 Service Manual (74 pages)

Brand: Digital | Category: Multifunctionals | Size: 0.16 MB |

English

Table of contents

Compaq DECprint Supervisor

1

(DCPS)forOpenVMS

1

ReleaseNotes

1

Contents

3

4 Printer-Speciﬁc Information

4

A Raw TCP/IP Port Numbers

8

Introduction

9

Release Notes Contents

9

SPD Contents

9

DCPS Version 2.0 Information

11

1.2 DCPS Version 2.0 Fixes

12

DCPS Restrictions

13

2–2 DCPS Restrictions

14

DCPS Restrictions 2–3

15

2–4 DCPS Restrictions

16

DCPS Restrictions 2–5

17

2–6 DCPS Restrictions

18

Printers

20

Printer-Speciﬁc Information

23

4.5 Tektronix Phaser

24

DCPS Version 1.8 Information

25

6.2 DCPS Version 1.7-1 Fixes

29

DCPS Version 1.7 Information

31

8.2 DCPS Version 1.6-1 Fixes

33

DCPS Version 1.6 Information

35

10.1 DCPS Version 1.5-2 Fixes

39

11.2 DCPS Version 1.5-1 Fixes

41

DCPS Version 1.5 Information

43

DCPS Version 1.4 Information

45

13.3 DCPS Version 1.4 Changes

47

13.3.5 New Paper Sizes

48

13.3.9 DCPS User Card Removed

49

DCPS Version 1.3 Information

51

Parameters

52

14.3 DCPS Version 1.3 Changes

53

DCPS Version 1.2 Information

55

15.2 DCPS Version 1.2 Fixes

56

15.3 DCPS Version 1.2 Changes

58

DCPS Version 1.1A Information

61

DCPS Version 1.1 Information

63

17.2 DCPS Version 1.1 Fixes

64

17.3 DCPS Version 1.1 Changes

66

17.3.3 LN03 Image Printer

67

Raw TCP/IP Port Numbers

69

Digital LSR 2000 User Manual (111 pages)

Brand: Digital | Category: Multifunctionals | Size: 4.54 MB |

English

Table of contents

Droplet Digital

1

Applications Guide

1

Table of Contents

2

Table of Contents

4

1 Droplet Digital

5

QX100/QX200 Workﬂow

6

PCR Ampliﬁcation

8

Experimental Considerations

11

8

12

2 Designing Droplet

15

Designing Probes

16

Designing an Assay

17

14

18

Designing Droplet Digital

18

PCR Experiments

18

Sample Preparation

19

16

20

Droplet Generation

21

Software

23

Droplet Reading

24

Data Analysis

24

Merging Wells

26

EvaGreen and Gene Expression

30

Reference

31

3 Absolute Quantiﬁcation

32

Statistics of ddPCR

34

Copies per Microliter

35

Copies per Droplet

35

Low Concentration Example

36

High Concentration Example

37

Deﬁnitions

38

Errors in ddPCR

40

4 Copy Number

41

38

42

CNV Calculations

43

Assay Design

44

Running a CNV Assay

45

Restriction Digestion

45

42

46

BSA, bovine serum albumin

47

5 Rare Mutation and

49

46

50

Rare Mutation Detection

51

Testing an RMD Assay

52

RMD Experiment Considerations

52

Recommended Controls

56

Rare Sequence Detection

57

RSD Experimental Strategies

58

References

60

6 Gene Expression

61

Obtain RNA

62

Generate cDNA

62

Data Analysis Results

64

Gene Expression

65

7 Next-Generation

68

Decreasing size of library

70

Adapter dimers

70

Library Balancing

72

1 2 3 4 5 6 7 8 9 10 11 12

73

70

74

Chloroform Aqueous

74

8 Additional Applications

76

Milepost Assay

77

74

78

Additional Applications

78

Multiplexing

79

9 Droplet Digital

82

No or Few Positive Droplets

87

No or Low Total Droplet Count

87

Insufﬁcient Mixing

88

86

90

Target Accessibility

91

88

92

– + – + – +

94

Appendix A

95

Ordering Information

95

92

96

Appendix B

98

Technical Error Bars

98

Partitioning

100

Appendix C

102

Acronyms

102

Appendix C: Acronyms

103

Index

104

102

106

PCR Applications Guide

106

104

108

106

110

Life Science

111

Bio-Rad

111

Laboratories, Inc

111

Digital LSR 2000 User's Guide (7 pages)

Brand: Digital | Category: Multifunctionals | Size: 0.03 MB |

English

Table of contents

Colorwriter LSR 2000 Printer

1

Release Notes Version 2.1

1

Related documentation

2

New features in v2.1 software

2

Supported Platforms

3

Take note

4

Printing

5

Low Toner

5

Power Saver Mode

5

Updating System Software

6

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